全文获取类型
收费全文 | 2800篇 |
免费 | 142篇 |
国内免费 | 232篇 |
出版年
2024年 | 3篇 |
2023年 | 28篇 |
2022年 | 46篇 |
2021年 | 32篇 |
2020年 | 61篇 |
2019年 | 67篇 |
2018年 | 108篇 |
2017年 | 64篇 |
2016年 | 67篇 |
2015年 | 57篇 |
2014年 | 150篇 |
2013年 | 315篇 |
2012年 | 105篇 |
2011年 | 140篇 |
2010年 | 121篇 |
2009年 | 147篇 |
2008年 | 146篇 |
2007年 | 173篇 |
2006年 | 150篇 |
2005年 | 127篇 |
2004年 | 88篇 |
2003年 | 107篇 |
2002年 | 104篇 |
2001年 | 97篇 |
2000年 | 90篇 |
1999年 | 75篇 |
1998年 | 41篇 |
1997年 | 30篇 |
1996年 | 29篇 |
1995年 | 48篇 |
1994年 | 43篇 |
1993年 | 38篇 |
1992年 | 38篇 |
1991年 | 22篇 |
1990年 | 11篇 |
1989年 | 16篇 |
1988年 | 12篇 |
1987年 | 12篇 |
1986年 | 12篇 |
1985年 | 19篇 |
1984年 | 24篇 |
1983年 | 17篇 |
1982年 | 14篇 |
1981年 | 18篇 |
1980年 | 12篇 |
1979年 | 14篇 |
1978年 | 10篇 |
1977年 | 12篇 |
1976年 | 6篇 |
1973年 | 4篇 |
排序方式: 共有3174条查询结果,搜索用时 31 毫秒
1.
《Bioorganic & medicinal chemistry letters》2014,24(10):2329-2333
The fruits of Cudrania tricuspidata (Carr.) Bur. (Moraceae) significantly inhibited pancreatic lipase, which plays a key role in fat absorption. Optimization of extraction conditions with minimum pancreatic lipase activity and maximum yield was determined using response surface methodology with three-level-three-factor Box–Behnken design (BBD). Regression analysis showed a good fit of the experimental data and the optimal condition was obtained as ethanol concentration, 74.5%; temperature 61.9 °C and extraction time, 13.5 h. The pancreatic lipase activity and extraction yield under optimal conditions were found to be 65.5% and 54.0%, respectively, which were well matched with the predicted value of 65.8% and 47.1%. Further fractionation of C. tricuspidata extract resulted in the isolation of compound 1, which was identified as 5,7,4′-trihydroxy-6,8-diprenylisoflavone. It inhibited pancreatic lipase activity with IC50 value of 65.0 μM. HPLC analysis suggested positive correlation between pancreatic lipase inhibition and 5,7,4′-trihydroxy-6,8-diprenylisoflavone of C. tricuspidata fruits. 相似文献
2.
3.
J. L. L. Rakels A. G. M. Schneiders A. J. J. Straatho J. J. Heijnen 《Biocatalysis and Biotransformation》1996,13(3):179-188
Competitive parallel reactions with opposite enantioselectivity are presented as a strategy to enhance the enantiomeric product purity in enzymatic kinetic resolution. Lipase-catalyzed simultaneous hydrolysis and amidation of racemic methy 12-chloropropionate led to significantly improved amide yield and enantiomeric excess. Process results can be controlled by changing the hydrolysis/amidation reaction rates through variation of the solvent and the initial amine concentration. This is described by a kinetic model. 相似文献
4.
X Y Wu Y-Y Linko J Seppälä M Leisola P Linko 《Journal of industrial microbiology & biotechnology》1998,20(6):328-332
The enzymatic synthesis of aromatic polyesters by direct polyesterification between a diacid and a diol is described. The
effects of the type of substrate, type and quantities of lipase, temperature, vacuum, and reaction time on the synthesis of
aromatic polyesters were studied in detail. Among three lipases investigated, only Novozym 435 worked well for aromatic polyester
synthesis. Temperature and vacuum played an important role in obtaining a high molar mass of the aromatic polyesters. Furthermore,
with isophthalic acid and 1,6-hexanediol as substrates, the mass average molar mass of the polyester obtained increased with
an increase in the lipase quantity up to 0.375 g (11.7%, w/w of total reactor contents). The mass average molar mass of the
polyester was as high as 50000 g mol−1 in 168 h, with a polydispersity of PD ≈ 1.4.
Received 27 January 1998/ Accepted in revised form 19 May 1998 相似文献
5.
Triglyceride-lipase (TGL) is a major fat body lipase in Manduca sexta. The knowledge of how TGL activity is regulated is very limited. A WWE domain, presumably involved in protein–protein interactions, has been previously identified in the N-terminal region of TGL. In this study, we searched for proteins partners that interact with the N-terminal region of TGL. Thirteen proteins were identified by mass spectrometry, and the interaction with four of these proteins was confirmed by immunoblot. The oxidoreductase lipoamide-dehydrogenase (LipDH) and the apolipoprotein components of the lipid transporter, HDLp, were among these proteins. LipDH is the common component of the mitochondrial α-keto acid dehydrogenase complexes whereas HDLp occurs in the hemolymph. However, subcellular fractionation demonstrated that these two proteins are relatively abundant in the soluble fraction of fat body adipocytes. The cofactor lipoate found in typical LipDH substrates was not detected in TGL. However, TGL proved to have critical thiol groups. Additional studies with inhibitors are consistent with the notion that LipDH acting as a diaphorase could preserve the activity of TGL by controlling the redox state of thiol groups. On the other hand, when TG hydrolase activity of TGL was assayed in the presence of HDLp, the production of diacylglycerol (DG) increased. TGL-HDLp interaction could drive the intracellular transport of DG. TGL may be directly involved in the lipoprotein assembly and loading with DG, a process that occurs in the fat body and is essential for insects to mobilize fatty acids. Overall the study suggests that TGL occurs as a multi-protein complex supported by interactions through the WWE domain. 相似文献
6.
Hiroyuki Akita 《Biocatalysis and Biotransformation》1996,13(3):141-156
Water-insoluble compounds can be substrates for enzymatic reactions when lipases are immobilized properly and suitable organic solvents are used. In this review, three type of lipase immobilization method and their application to the asymmetric syntheses of complex molecules are described. Lipases immobilized with Celite or synthetic prepolymers such as urethane prepolymer and photo-crosslinkable resin prepolymer have been applied for the kinetic resolution of many kinds of water-insoluble substrate.
Phospholipid-lipase aggregates with ether linkages are novel and have been found to function effectively as immobilized lipases in asymmetric hydrolysis or esterification reactions in water-saturated organic solvent. The phospholipid-lipase aggregates are considered to have a stacked bilayer based on X-ray diffraction analysis structure of the lipid in the crystalline phase. 相似文献
Phospholipid-lipase aggregates with ether linkages are novel and have been found to function effectively as immobilized lipases in asymmetric hydrolysis or esterification reactions in water-saturated organic solvent. The phospholipid-lipase aggregates are considered to have a stacked bilayer based on X-ray diffraction analysis structure of the lipid in the crystalline phase. 相似文献
7.
Ontogeny of lipase expression in winter flounder 总被引:3,自引:0,他引:3
H. M. Murray J. W. Gallant J. C. Perez-Casanova S. C. Johnson S. E. Douglas 《Journal of fish biology》2003,62(4):816-833
The partial sequencing of two lipases from winter flounder Pseudopleuronectes americanus , one most closely related to gastric, lingual and lysosomal acid lipase from other vertebrates and one most closely related to bile salt-activated lipase, is reported. Biochemical analyses of enzymatic activity demonstrated the greater contribution made by bile salt-activated lipase relative to neutral bile salt-independent lipase. Using molecular techniques, the tissue-specific expression of bile salt-activated lipase in pancreatic tissue and acid triacylglycerol lipase in a wide variety of organs was demonstrated. Furthermore, the developmental expression of these types of lipase in larval fish was established. 相似文献
8.
Marco Filice Marzia Marciello Lorena Betancor Alfonso V. Carrascosa Jose M. Guisan Gloria Fernandez‐Lorente 《Biotechnology progress》2011,27(4):961-968
Rhizomucor miehei lipase (RML) is greatly hyperactivated (around 20‐ to 25‐fold toward small substrates) in the presence of sucrose laurate. Hyperactivation appears to be an intramolecular process because it is very similar for soluble enzymes and covalently immobilized derivatives. The hyperactivated enzyme was immobilized (in the presence of sucrose laurate) on cyanogen bromide‐activated Sepharose (very mild covalent immobilization through the amino terminal residue), on glyoxyl Sepharose (intense multipoint covalent immobilization through the region with the highest amount of Lys residues), and on different anion exchangers (by multipoint anionic exchange through the region with the highest density of negative charges). Covalent immobilization does not promote the fixation of the hyperactivated enzyme, but immobilization on Sepharose Q retains the hyperactivated enzyme even in the absence of a detergent. The hydrolysis of fish oils by these hyperactivated enzyme derivatives was sevenfold faster than by covalently immobilized derivatives and three and a half times faster than by the enzyme hyperactivated on octyl‐Sepharose. The open structure of the hyperactivated lipase is fairly exposed to the medium, and no steric hindrance should interfere with the hydrolysis of large substrates. These new hyperactivated derivatives seem to be more suitable for hydrolysis of oils by RML immobilized inside porous supports. In addition, the hyperactivated derivatives are fairly stable against heat and organic cosolvents. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011 相似文献
9.
Neurospora crassa Em 5297a can utilize sodium Β-glycerophosphate as a sole phosphorous source (in the place of KH2PO4). Under these conditions
a repressible alkaline phosphatase is elaborated which has different pH optimum towards Β-glycerophosphate (10.2) and pyrophosphate
(9.0) as substrates. This enzyme does not require any metal ion for its activity and could be assayed in the presence of EDTA.
However, under conditions of cobalt toxicity, the activity of this enzyme is high and is decreased in copper and nickel toxicities. 相似文献
10.